Facts-enriched characterization of mobile populations. Morphological information from illustrations or photos can incorporate for the richness of flow cytometry knowledge. By way of example, the figure shows an or else traditional apoptosis assay utilizing Annexin V and PI, incorporating mobile imaging to characterize cells in Just about every population to expose morphologically unique capabilities. These insights could not have been received from multiplex staining on your own.
As samples are obtained to the Attune CytPix Flow Cytometer, the high-pace brightfield digicam captures and stores visuals of detected functions in a price as many as six,000 illustrations or photos for every second, according to the flow level and graphic size. For higher flexibility, Attune Cytometric Computer software permits you to alter the graphic capture frequency as essential and choose a selected gate for impression capture, if expected.
Combine acoustic focusing technological innovation with superior-parameter Assessment for adaptable spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a large-pace brightfield digital camera for simultaneous high throughput flow cytometry and large resolution brightfield imaging.
"An image just isn't fairly really worth one thousand dots, nonetheless it definitely helps to see images. It is excellent to see pictures that correlate with staining. For instance, AnnexinV-optimistic cells which are morphologically distinctive than nutritious cells, or CD14+ monocytes which have been bigger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a skilled person has gated singlets confidently. Right after analyzing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate incorporates much more than 4% aggregates.
Pair your Attune flow cytometer with an autosampler for enhanced workflow effectiveness, and add robotic integration for maximum throughput.
Our scientists will style and design panels for you personally as many times you would like—cost-free, no products acquire necessary.
Early indications of this contamination experienced in the beginning been dismissed as debris. Backgating to find out new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also permits you to use morphological functions to discover interesting subpopulations that could not be evident from flow cytometry details by itself.
Most likely most significantly, these gatherings include cells of Evidently unique phenotypes which may lead to incorrect conclusions about double favourable situations (specifically in rare populations).
AI-pushed automatic image Investigation Together with the Attune Cytometric Software package translates event capabilities into distinct morphology parameters which might be combined with standard fluorescence and scatter parameters to expose remarkably reproducible population variations and exceptional subsets.
Software Notice: Automated impression analysis minimizes person-to-person variability in flow cytometry gating procedures
Leverage the power of Innovative engineering and innovation to accelerate your discoveries. Trust within the Attune Flow Cytometer loved ones to deliver the dependability, pace, and accuracy that established your function apart.
No wash/no lyse leukocyte analysis with imaging. Cells were being acquired from 24-hour-old blood by dilution in one mM EDTA ( Skilled UX/UI allows buyers to teach AI designs for expanded apps. (A) The “Prepare Design” feature gives annotation resources and genuine-time product schooling accuracy.
Gating on these prolonged parameters helps you to swiftly and precisely detect populations of interest and ensure gating strategy with little if any guide evaluation. The Attune computer software features usage of condition-of-the-art equipment Discovering Assessment equipment in an expertly intended person interface, making it exceptionally simple to use.
Since the cell line had Earlier been analyzed over the Attune CytPix Flow Cytometer, the scientists went back again more info to the pictures and have been able to doc the microbial an infection at the very least five days previously.
The Attune CytPix Flow Cytometer permits consumers to visualise samples for the duration of acquisition, validate the precision of gating techniques, and assistance results with reliable documentation for publication uses.
Right here, we exhibit that by gating tactics working with impression-primarily based quantitative parameters can distinguish interacting cells from coincident occasions to a lot more precisely analyze interacting cells.