At that time, the early signals were dismissed as debris, although the retrospective analysis shown shared traits Together with the problematic cells in culture. Tracing the infection helped the lab establish additional laboratory treatments for screening and safety of assay-critical mobile strains.
Irrespective of whether you’re a starter or an expert, take a look at our methods to advance your study and experience in flow cytometry.
(B) RAW 264.7 macrophages are sometimes annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The number of singlets readily available for analysis increases after the consumer trains and employs a more correct design.
Discover new insights into your experimental information by visualizing populations applying combined fluorescence and morphology facts. Our software package interface wizards empower downsampling, concatenation (merged documents) and dimensionality instruments that are constructed to the software program for an intuitive, streamlined details Assessment working experience.
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Below, an experienced person has gated singlets confidently. Soon after evaluating the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate has over four% aggregates.
Pair your Attune flow cytometer by having an autosampler for elevated workflow efficiency, and incorporate robotic integration for max throughput.
Consistent picture excellent even at significant throughput Acoustic concentrating as well as a superior-speed digital camera Blend to graphic these T cells consistently at minimal or significant flow charges. Very easily modify concentration and camera settings to meet experimental demands.
Early indications of this contamination experienced in the beginning been dismissed as debris. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also allows you to use morphological characteristics to find fascinating subpopulations that might not be evident from flow cytometry details alone.
End users can look at occasion images in authentic-time to verify sample good quality, improve protocols, and report the morphology of every cell population identified for Examination. Imaging seize also Advantages from acoustic concentrating, which helps you to placement the cells so that a pointy, centered graphic is obtained.
Audio waves help specific cell alignment, leading to Outstanding info quality and ~10X more rapidly sample flow prices.
We Earlier shown the power of imaging CAR-T/Ramos cell interactions. Enable’s evaluate just the inhabitants of best interest, the double positive functions, to learn more. We can easily now use prolonged morphological parameters (here, circularity and skewness of depth) to more examine the characteristics of those populations and refine gating on these occasions, escalating data robustness.
Leverage the power of advanced technology and innovation to speed up your discoveries. Rely on in the Attune Flow Cytometer family to provide the trustworthiness, pace, and precision that set your operate aside.
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Gating on these prolonged parameters enables you to swiftly and precisely determine populations of fascination and make sure gating system with little or no manual critique. The Attune application provides use of condition-of-the-art equipment Discovering Assessment instruments inside an expertly developed consumer interface, making it exceptionally easy to use.
The Attune Xenith cytometer is actually a substantial-parameter spectral analyzer, delivering both of those spectral unmixing and conventional flow abilities for comprehensive and functional mobile Examination.
The Attune CytPix Flow Cytometer permits users to visualize samples throughout acquisition, verify the precision of gating procedures, and guidance outcomes with dependable documentation for publication functions.
Listed here, we exhibit that by gating tactics using picture-based mostly quantitative parameters can distinguish interacting cells from coincident situations to far more properly analyze interacting cells.